Whole Blood Processing Using the Buffy-Coat Method
نویسندگان
چکیده
منابع مشابه
Evaluation of buffy coat 16S rRNA PCR, buffy coat culture and whole blood PCR for detection of bacteraemia.
The use of Gram type-specific PCR on buffy coat from clinical specimens for the detection of bacteraemia was evaluated for the first time using whole blood culture as the gold standard. In addition, the established buffy coat culture and whole blood PCR were also compared. Gram-positive bacteria belonging to six species and Gram-negative bacteria from 10 species were isolated and identified by ...
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Cord blood DNA methylation is associated with numerous health outcomes and environmental exposures. Whole cord blood DNA reflects all nucleated blood cell types, while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. Both sample types are used in DNA methylation studies. Cell types have unique methylation patterns and processing can impact cell distr...
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The elimination of a thorough manual mixing of whole blood (WB) which takes place following the overnight hold, but before the first centrifugation step, during buffy coat component production at Canadian Blood Services (CBS) was investigated. WB was pooled after donation and split. Pairs of platelet, red blood cell (RBC), and plasma components were produced, with half using the standard method...
متن کاملPreparation and pathogen inactivation of double dose buffy coat platelet products using the INTERCEPT blood system.
Blood centers are faced with many challenges including maximizing production yield from the blood product donations they receive as well as ensuring the highest possible level of safety for transfusion patients, including protection from transfusion transmitted diseases. This must be accomplished in a fiscally responsible manner which minimizes operating expenses including consumables, equipmen...
متن کاملBuffy Coat in Diagnosis of Pulmonary Tuberculosis
Background: Besides charging the patients with outstanding costs, tuberculosis (TB) causes high mortality and morbidity in a country. We studied the sensitivity, specificity, positive and negative predictive values as well as the efficiency of buffy coat smear test in patients who were pulmonary TB suspects. This research was conducted at Massih Daneshvari Hospital, National Research Institute ...
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ژورنال
عنوان ژورنال: The Korean Journal of Blood Transfusion
سال: 2020
ISSN: 1226-9336,2383-6881
DOI: 10.17945/kjbt.2020.31.3.201